| Date | PIX Time | Match | Round | Venue |
|---|
| 28-Nov-09 | | Northwich Victoria 1 - 3 Lincoln City | Round 2 | Marston Arena |
| 28-Nov-09 | | Staines Town 1 - 1 Millwall | Round 2 | Wheatsheaf Park |
| 28-Nov-09 | | Accrington Stanley 2 - 2 Barnet | Round 2 | The Fraser Eagle Stadium |
| 28-Nov-09 | | AFC Bournemouth 1 - 2 Notts County | Round 2 | The Fitness First Stadium |
| 28-Nov-09 | | Bath 1 - 2 Forest Green Rovers | Round 2 | Twerton Park |
| 28-Nov-09 | | Brentford 1 - 0 Walsall | Round 2 | Griffin Park |
| 28-Nov-09 | | Brighton 3 - 2 Rushden & Diamonds | Round 2 | Withdean Stadium |
| 28-Nov-09 | | Cambridge United 1 - 2 York City | Round 2 | The Trade Recruitment Stadium |
| 28-Nov-09 | | Gillingham 1 - 0 Burton Albion | Round 2 | KRBS Priestfield Stadium |
| 28-Nov-09 | | Hereford United 0 - 1 Colchester United | Round 2 | Edgar Street |
| 28-Nov-09 | | Milton Keynes Dons 4 - 3 Exeter City | Round 2 | stadium:mk |
| 28-Nov-09 |  15:00 (IST) | Northampton Town 2 - 3 Southampton | Round 2 | Sixfields Stadium |
| 28-Nov-09 | | Oxford United 1 - 1 Barrow | Round 2 | The Kassam Stadium |
| 28-Nov-09 | | Port Vale 0 - 1 Huddersfield Town | Round 2 | Vale Park |
| 28-Nov-09 | | Rotherham United 2 - 2 Luton Town | Round 2 | Don Valley Stadium |
| 28-Nov-09 | | Stockport County P - P Torquay United | Round 2 | Edgeley Park |
| 28-Nov-09 | | Tranmere Rovers 0 - 0 Aldershot Town | Round 2 | Prenton Park |
| 28-Nov-09 | | Wrexham 0 - 1 Swindon Town | Round 2 | Racecourse Ground |
| 28-Nov-09 | | Carlisle United 3 - 1 Norwich City | Round 2 | Brunton Park |
| 29-Nov-09 | 13:45 (IST) | Kettering Town 1 - 1 Leeds United | Round 2 | Elgood's Brewery Arena |
| 8-Dec-09 | | Aldershot Town 1 - 2 Tranmere Rovers | Round 2 | The EBB Stadium at the Recreation Ground |
| 8-Dec-09 | | Barnet 0 - 1 Accrington Stanley | Round 2 | Underhill Stadium |
| 8-Dec-09 | | Barrow 3 - 1 Oxford United | Round 2 | Holker Street |
| 8-Dec-09 | | Luton Town 3 - 0 Rotherham United | Round 2 | Kenilworth Road |
| 8-Dec-09 | | Stockport County P - P Torquay United | Round 2 | Edgeley Park |
| 8-Dec-09 | | Leeds United 5 - 1 Kettering Town | Round 2 | Elland Road |
| 9-Dec-09 | | Millwall 4 - 0 Staines Town | Round 2 | The New Den |
| 15-Dec-09 | | Stockport County 0 - 4 Torquay United | Round 2 | The Moss Rose Ground |
