| Date | PIX Time | Match | Round | Venue |
|---|
| 6-Nov-09 | | Bristol Rovers 2 - 3 Southampton | Round 1 | The Memorial Stadium |
| 6-Nov-09 | | Huddersfield Town 6 - 1 Dagenham & Redbridge | Round 1 | The Galpharm Stadium |
| 6-Nov-09 | | Notts County 2 - 1 Bradford City | Round 1 | Meadow Lane |
| 7-Nov-09 | | Bromley 0 - 4 Colchester United | Round 1 | The Courage Stadium |
| 7-Nov-09 | | Stourbridge 0 - 1 Walsall | Round 1 | War Memorial Athletic Ground |
| 7-Nov-09 | | Accrington Stanley 2 - 1 Salisbury City | Round 1 | The Fraser Eagle Stadium |
| 7-Nov-09 | | AFC Telford United 1 - 3 Lincoln City | Round 1 | New Bucks Head Stadium |
| 7-Nov-09 | | Aldershot Town 2 - 0 Bury | Round 1 | The EBB Stadium at the Recreation Ground |
| 7-Nov-09 | | Barnet 3 - 1 Darlington | Round 1 | Underhill Stadium |
| 7-Nov-09 | | Barrow 2 - 1 Eastleigh | Round 1 | Holker Street |
| 7-Nov-09 | | Cambridge United 4 - 0 Ilkeston Town | Round 1 | The Trade Recruitment Stadium |
| 7-Nov-09 | | Carlisle United 2 - 2 Morecambe | Round 1 | Brunton Park |
| 7-Nov-09 | | Chesterfield 1 - 3 AFC Bournemouth | Round 1 | Recreation Ground |
| 7-Nov-09 | | Forest Green Rovers 1 - 1 Mansfield Town | Round 1 | The New Lawn |
| 7-Nov-09 | | Gateshead 2 - 2 Brentford | Round 1 | The International Stadium |
| 7-Nov-09 | | Gillingham 3 - 0 Southend United | Round 1 | KRBS Priestfield Stadium |
| 7-Nov-09 | | Grimsby Town 0 - 2 Bath | Round 1 | Blundell Park |
| 7-Nov-09 | | Hartlepool United 0 - 1 Kettering Town | Round 1 | Victoria Park |
| 7-Nov-09 | | Hereford United 2 - 0 Sutton United | Round 1 | Edgar Street |
| 7-Nov-09 | | Luton Town 3 - 3 Rochdale | Round 1 | Kenilworth Road |
| 7-Nov-09 | | Milton Keynes Dons 1 - 0 Macclesfield Town | Round 1 | stadium:mk |
| 7-Nov-09 | | Northampton Town 2 - 1 Fleetwood Town | Round 1 | Sixfields Stadium |
| 7-Nov-09 | | Nuneaton 0 - 4 Exeter City | Round 1 | Manor Park |
| 7-Nov-09 | | Oxford United 1 - 0 Yeovil Town | Round 1 | The Kassam Stadium |
| 7-Nov-09 | | Port Vale 1 - 1 Stevenage Borough | Round 1 | Vale Park |
| 7-Nov-09 | | Rushden & Diamonds 3 - 1 Hinckley Utd | Round 1 | Nene Park |
| 7-Nov-09 | | Shrewsbury Town 0 - 1 Staines Town | Round 1 | Prostar Stadium |
| 7-Nov-09 | | Stockport County 5 - 0 Tooting & Mitcham | Round 1 | Edgeley Park |
| 7-Nov-09 | | Swindon Town 1 - 0 Woking | Round 1 | County Ground |
| 7-Nov-09 | | Torquay United 3 - 1 Cheltenham Town | Round 1 | Plainmoor Ground |
| 7-Nov-09 | | Tranmere Rovers 1 - 1 Leyton Orient | Round 1 | Prenton Park |
| 7-Nov-09 | | Wrexham 1 - 0 Lowestoft Town | Round 1 | Racecourse Ground |
| 7-Nov-09 | | Wycombe Wanderers 4 - 4 Brighton | Round 1 | Adams Park |
| 7-Nov-09 | | York City 3 - 2 Crewe Alexandra | Round 1 | KitKat Crescent |
| 7-Nov-09 | | Oldham Athletic 0 - 2 Leeds United | Round 1 | Boundary Park |
| 8-Nov-09 | | Northwich Victoria 1 - 0 Charlton Athletic | Round 1 | Marston Arena |
| 8-Nov-09 | | Burton Albion 3 - 2 Oxford City | Round 1 | Pirelli Stadium |
| 8-Nov-09 | | Wealdstone 2 - 3 Rotherham United | Round 1 | Grosvenor Vale |
| 9-Nov-09 | | Millwall 4 - 1 AFC Wimbledon | Round 1 | The New Den |
| 11-Nov-09 | | Rochdale 0 - 2 Luton Town | Round 1 | Spotland Stadium |
| 17-Nov-09 | | Brentford 5 - 2 Gateshead | Round 1 | Griffin Park |
| 17-Nov-09 | | Leyton Orient 0 - 1 Tranmere Rovers | Round 1 | Matchroom Stadium |
| 17-Nov-09 | | Mansfield Town 1 - 2 Forest Green Rovers | Round 1 | Field Mill Ground |
| 17-Nov-09 | | Morecambe 0 - 1 Carlisle United | Round 1 | Christie Park |
| 17-Nov-09 | | Stevenage Borough 0 - 1 Port Vale | Round 1 | Broadhall Way |
| 18-Nov-09 | | Brighton 2 - 0 Wycombe Wanderers | Round 1 | Withdean Stadium |
